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Final purified mitochondrial fractions stained with BioTracker 633 Red Mitochondria Dye (middle) and TOM20 (left) show viable pure mitochondria (merged; right). Scale bar is 5 µm.

Journal: MethodsX

Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method ☆

doi: 10.1016/j.mex.2026.103871

Figure Lengend Snippet: Final purified mitochondrial fractions stained with BioTracker 633 Red Mitochondria Dye (middle) and TOM20 (left) show viable pure mitochondria (merged; right). Scale bar is 5 µm.

Article Snippet: This method is based on Mitochondria Extraction Kit – Tissue and Mitochondria Isolation Kit mouse tissue, and their respective protocols by Miltenyi Biotec, with adjusted centrifugation conditions and added washing steps on final purified fractions.

Techniques: Purification, Staining

Final purified mitochondrial fractions stained with BioTracker 633 Red Mitochondria Dye (middle) and TOM20 (left) show viable pure mitochondria (merged; right). Scale bar is 5 µm.

Journal: MethodsX

Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method ☆

doi: 10.1016/j.mex.2026.103871

Figure Lengend Snippet: Final purified mitochondrial fractions stained with BioTracker 633 Red Mitochondria Dye (middle) and TOM20 (left) show viable pure mitochondria (merged; right). Scale bar is 5 µm.

Article Snippet: Mitochondria Extraction Kit – Tissue (Miltenyi Biotec, Cat#130-097-340) Mitochondria Isolation Kit, mouse tissue (Miltenyi Biotec, Cat#130-096-946) Myelin Removal Beads II, human, mouse, rat (Miltenyi Biotec, Cat#130-096-433) Dulbecco’s PBS (1X), without Ca and Mg, without Phenol Red (Capricorn Scientific, Cat#PBS-1A) Roche cOmpleteTM, Mini, EDTA-free Protease Inhibitor Cocktail (Roche, Cat#11836170001) Bovine Serum Albumin heat shock fraction, pH 7, ≥ 98 % (Sigma-Aldrich, Cat#A7906) BioTracker 633 Red Mitochondria Dye (Sigma-Aldrich, Cat#SCT137) SuperSignalTM West Femto Maximum Sensitivity Substrate (Thermo Scientific, Cat#34096) PierceTM BCA Protein Assay Kit (Thermo Scientific, Cat#23227)

Techniques: Purification, Staining